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Month: July 2024

Appreciating the tinies

Posted on 2024-07-022024-07-02 by Allison J. Gong

Earlier this week I collected a plankton sample and settled down for a day of microscopy. For a variety of reasons it was my first foray into actual biology for the month of June, and I just wanted to feel like a marine biologist for a while.

As far as plankton samples go, there wasn’t a lot to write home about. The large centric diatoms that we had seen in the spring were much less abundant, although there was quite a bit of the pennate diatom Pseudonitzschia. Part of the reason I did the plankton tow was to have something to look at under the microscope and to practice taking photos. There are all sorts of gadgets that allow one to use a phone to take photos through the microscope, but I’ve found those to be either specific to one phone model or too fiddly and frustrating to get properly lined up. Besides, when I bought my microscope several years ago now I had the foresight to splurge for the trinocular head, which allows me to mount a real camera and leaves both eyepieces available to look through. Might as well take advantage of it!

So, I just took a bunch of photos.

First up was a chain diatom in the genus Chaetoceros. Phase contrast lighting might not have been the best option here, but oh well. Chaetoceros cells are box-shaped, with a spine protruding from each corner of the box. Aside: ‘chaeto’ means ‘hair’ or ‘bristle’ in Greek. The spines of adjacent cells sort of interlock and hold the cells together, forming the chain. Spines also provide some defense against predation.

Many species of Chaetoceros form straight chains like this.

Chain diatom Chaetoceros sp., viewed with phase contrast lighting
2024-06-24
©Allison J. Gong

But one species, Chaetoceros debilis, forms spiral chains!

Spiral chain of gold-colored boxes
Chain diatom Chaetoceros debilis, viewed with brightfield lighting
2024-06-24
©Allison J. Gong

Earlier in the spring there were a lot of Coscinodiscus diatoms in the local plankton. Those are the big button-like diatoms with the sculpted frustule. They aren’t nearly as common now, but I did see a few. And managed to get a nice shot of one:

Golden circular object

The star of the show was Thalassiothrix, another diatom in which the ends of cells cells remain connected after dividing. Instead of forming chains as Chaetoceros does, Thalassiothrix makes colonies that are either zig-zag or star-shaped. It just so happens that this organism looks especially brilliant under darkfield lighting, so I was very happy.

Diatom Thalassiothrix sp, viewed under darkfield lighting
2024-06-24
©Allison J. Gong

But take a look at this symmetry:

Fan-shaped arrangement of long golden rectangles, against a black background
Diatom Thalassiothrix sp, viewed under darkfield lighting
2024-06-24
©Allison J. Gong

Isn’t that a spectacular organism? I had a lot of fun developing and processing that image, and am happy at how well it turned out. Darkfield lighting is fun to play with!

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